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Fisher Scientific S90257 - Edvotek™ Wester… | USA MedPremium
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Western Blot Educational Kit
Edvotek™ Western Blot Educational Kit 6 blots, 2.5 hours Time Range, Refrigerated
USAMP#90754957
SKU: S90257
Edvotek®
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Proteins are separated on a polyacrylamide gel and are transferred (blotted) to a nylon membrane
Membrane is exposed to solutions containing primary antibody, followed by a secondary antibody coupled to an enzyme
Membrane is then soaked in a substrate solution to develop the color reaction to identify the antigen protein band
Molecular weights of the visible bands are measured using prestained protein markers of known molecular weight
Experiment times: Electrophoresis, one hour, blot, overnight, detection, two hours and 30 minutes
Includes: Instructions, negative control, all samples and antibodies, various reagents and buffers, membrane and filter paper
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