Provides results in less than 30 minutes no overnight cultures required
Compatible with primary fecal samples, broth, plate cultures and specimens in transport media
Employs rapid membrane enzyme immunoassay
Membrane device contains reaction window with two vertical lines of immobilized antibodies for Stx1 and Stx2, plus anti-horseradish peroxidase (HRP) control line
Sample is mixed with diluent and conjugate and applied to sample well
Stx1 and Stx2 in sample bind to antibody-peroxidase conjugates and are subsequently captured by immobilized Stx1 and Stx2 specific monoclonal antibodies in test lines
Positive control reaction is indicated by vertical dotted blue line
